Objective The paper was conducted to estimate the breeding values of dairy cow reproductive characters and to compare the accuracy of different estimate methods of breeding values.

目的奶牛繁殖性状的育种值估计及比较不同育种值估计方法的准确性。

In which 30 Holstein dairy cattle were conducted with feed comparison trial, the experiment time is 30 days. The milk production of the group increased 3.91% (per day per dairy cow).

对30头荷斯坦泌乳牛在相同饲养条件下进行了30天饲养对比试验，与对照组相比试验组平均日产奶量增加了3.91％。

Dairy cow mammary gland epithelial cells cultured in vitro can be used for research of mammary gland development, lactation mechanism, and biological reactor applications etc.

体外培养的奶牛乳腺上皮细胞在乳腺生长发育和泌乳机制的研究及乳腺生物反应器的应用等方面具有重要作用。